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目的探讨血管紧张素原基因(GT)3′-端(GT)重复多态性与藏族原发性高血压(EH)易感相关性。方法导入基于群体的病例-对照相关分析。病例组360例(M/F=168/192);对照组380人(M/F=177/203)。平均年龄分别为51.4±14.5岁和47.8±12.4岁。应用荧光标记dctp参入PCR扩增和基因扫描技术检测各片段长度及基因型。结果藏族血管紧张素基因3′-端(GT)重复序列在10种主要等位基因(频率>1%),从小到大依次称为A1(162bp),A2,A……,A10(180bp)。重复次数依次为(GT)≥1.5,(GT)16……(GT)≥24。分布频率从1.7%～27.5%;A4(GT18)为常见等位基因。高血压组A7(GT21)频率较对照组明显升高(11.3%:5.9%,χ2=5.02,P=0.023,OR=2.01,95%CI:1.05～1.87)。结论 AGT基因3′-端(GT)n多态性与藏族EH易感相关,而与汉族EH无关联。
Objective To investigate the relationship between the polymorphism of 3’-terminal GT of angiotensinogen (GT) and susceptibility to Tibetan-essential hypertension (EH). Methods The population-based case-control correlation analysis was introduced. The case group was 360 (M / F = 168/192) and the control group was 380 (M / F = 177/203). The average age was 51.4 ± 14.5 years and 47.8 ± 12.4 years respectively. Fluorescent labeled dctp was used to detect the length and genotype of each fragment by PCR amplification and gene scanning. Results The sequence of the 3’-terminal (GT) repeats of the Tibetan angiotensin gene was designated as A1 (162bp), A2, A ..., A10 (180bp) in 10 major alleles (frequencies> 1%), . The order of repetition is (GT) ≥1.5, (GT) 16 ...... (GT) ≥24. Distribution frequency from 1.7% to 27.5%; A4 (GT18) is a common allele. The frequency of A21 (GT21) in hypertensive group was significantly higher than that in control group (11.3% vs 5.9%, χ2 = 5.02, P = 0.023, OR = 2.01, 95% CI: 1.05-1.87). Conclusion The 3’-terminal (GT) n polymorphism of AGT gene is associated with susceptibility to EH in Tibetan and no association with EH in Han.