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[目的]优化甘蔗试管苗瓶外生根的环境条件,提高成活率,降低生产成本。[方法]研究外界环境因素对甘蔗无根试管苗瓶外生根及生长的影响,比较瓶内和瓶外生根试管苗的成活率及株高差异。[结果]高肥力基质(如商品基质)处理的无根试管苗成活率低,但成活植株生长快,植株高,而低肥力基质(如新鲜河沙)更有利于无根试管苗生根成活,但植株生长慢;利用遮阳网和盖膜等措施调节温度、湿度和光照可使试管苗的生长环境逐渐过渡到自然环境条件;另外,瓶内生根试管苗的成活率和株高均显著高于瓶外生根试管苗。[结论]试管苗瓶外生根技术的研究成功有利于简化传统甘蔗试管苗生根技术,降低生产成本,促进甘蔗健康种苗的大面积推广种植。
[Objective] The research aimed to optimize the environmental conditions for rooting of sugarcane in vitro and increase the survival rate and reduce the production cost. [Method] The influence of external environmental factors on rooting and growth of non-root tube seedlings of sugarcane was studied. The survival rate and plant height difference of in vitro and in vitro rooting tube seedlings in bottle and bottle were compared. [Result] The survival rate of rootless test tube seedlings treated with high fertility matrix (such as commercial substrate) was low, but the survival plants grew fast and plants were high. However, the low fertility matrix (such as fresh river sand) But plant growth is slow. By adjusting the temperature, humidity and light, the growth environment of test-tube seedlings can be gradually transferred to the natural environment by measures such as shade net and cover film. In addition, the survival rate and plant height of test- Roots tube test tube plantlets. [Conclusion] The success of the study on rooting of vitro seedlings in vitro was in favor of simplifying the rooting technology of traditional sugarcane plantlets, reducing the production cost and promoting the popularization and planting of healthy seedlings of sugarcane.