论文部分内容阅读
目的 研究云南西双版纳傣族HBV感染人群基因型及基因亚型分布找出其优势基因型及基因亚型.方法 采用型特异性引物巢式PCR结合聚合酶链反应-限制性片段长度多态性法(PCR-nrLP)对HBV感染者基因型及亚型分型鉴定. 结果 B基因型58%(25/43),C基因型16%(7/43),B+C基因型21%(9/43),B+E型5%(2/43),无A、D和F型:Ba亚型100%(16/16),Cs亚型50%(2,4),Ce亚型50%(2t4). 结论 云南西双版纳傣族人群HBV基因型以B型为优势基因型Ba为优势基因亚型,国内首次发现B+E型,可能为研究其他民族HBV感染提供理论依据.“,”Objective To investigate the distribution of genotypes and subgenotypes of hepatitis B virus(HBY)among the population of Dai Nationality in Xishuangbanna，Yunnan Pmvinee.Methods The detection of HBV markers were conducted by enzyme-linked immunosorbnent assay(ELISA).HBV genotypes and subgenotypes of the serum samples were tested by PCR-RFLP. Results The genoty rnpe B was detected in 58%(25/43)of subjects，genotype C in 16%(7/43)、genotype B+C in 21%(9/43)、genotype B+E in5%(2/43).There were no genotypes of A、D and F.All of B genetypes blonged to subgenotype Ba and subgenotype Bj Wag not found.In 4 samples with genotype C，2(50%)were subgenotype Cs，2(50%)were subgenotypo Ce.Conclusion HBV genotype B is the predominant genotype and subgenotype Ba is the predominant subgenotyoo in Xishuangbanna.This is the first time to discover the B+E or E genotypes in China.This finding provides a new information for understanding the distribution of HBV genotype in China and a basis to establish Chinese gene bank.rn