目的:探讨HPPH光动力治疗对鼠G422脑胶质瘤脑及腋部皮下移植瘤各部位mtp53蛋白和p16蛋白表达的影响并和HpD-PDT做比较。方法:建立鼠G422脑胶质瘤脑及腋部皮下移植瘤模型,设立空白对照组、单注射HPPH 0.45mg/kg组、单注射HPD 5 mg/kg组、单665 nm激光照射组、单630n m激光照射组、HPPH-PDT各组(0.15 mg/kg组、0.3 mg/kg组、0.45 mg/kg组)、HpD-PDT 5 mg/kg组。单注射HPPH组、HPPH-PDT组和单注射HPD组、HpD-PDT组自尾静脉注入光敏剂,24 h后以波长665 nm的半导体激光照射HPPH-PDT组和单665 nm激光组肿瘤,功率密度200 mW/cm~2,每光斑照射17 min,能量密度为204 J/cm~2;以波长630 nm的半导体激光照射HpD-PDT组及单630 nm激光组肿瘤,功率密度200 mW/cm~2,每光斑照射20 min,能量密度为240 J/cm~2。于PDT后9 d处死鼠行酶联免疫吸附法(ELISA)的双抗体夹心法检测HPPH-PDT和HpD-PDT后肿瘤及对照组各部位(肿瘤、肿瘤边缘、正常脑组织)、血液突变型p53蛋白(mutant type p53 protein,mtp53)和p16蛋白表达变化。结果:空白、单注药和单照光三组对照组之间mtp53蛋白和p16蛋白表达值两两比较,P>0.05,差别无统计学意义。HPPH-PDT各剂量组及HPD-PDT组与空白对照组比较,mtp53蛋白表达值明显降低,p16蛋白表达值明显升高,P<0.01,差别有统计学意义。且接近正常脑组织值,或p16蛋白表达稍低于正常脑值,mtp53蛋白值表达稍高于正常脑组织值。0.3mg/kg和0.45mg/kg HPPH-PDT组与0.15 mg/kg HPPH-PDT组及HPD-PDT相比,mtp53蛋白表达值降低,p16蛋白表达值升高,P<005,差别有统计学意义,0.45 mg/kgHPPH-PDT组mtp53蛋白表达值稍低于0.3 mg/kgHPPH-PDT组,p16蛋白表达值稍高于0.3 mg/kgHPPH-PDT组,P>0.05,差别无统计学意义。HPPH-PDT0.3 mg/kg组的mtp53蛋白表达值低于HpD-PDT5 mg/kg组,p16蛋白表达值高于HpD-PDT 5 mg/kg组,P<0.05,差别有统计学意义。肿瘤边缘mtp53蛋白值稍高于肿瘤值,血液低于肿瘤值;p16蛋白值肿瘤边缘稍低于肿瘤值,血液高于肿瘤值。结论:HPPH-PDT能诱导mtp53蛋白表达降低,p16蛋白表达升高。与HpD-PDT相比,HPPH-PDTmtp53蛋白表达更低,p16蛋白表达更高。本次实验条件下HPPH 0.3 mg/kg是适宜的HPPH-PDT剂量。 OBJECTIVE: To investigate the effect of HPPH photodynamic therapy on the expression of mtp53 and p16 protein in the subcutaneous xenografts of brain and axillary tumors in mice with G422 glioma and to compare with that of HpD-PDT. Methods: The brain and axillary subcutaneous xenograft model of G422 glioma was established. A blank control group was established. Single injection of HPPH 0.45mg / kg group, single injection of HPD 5 mg / kg group, single 665 nm laser irradiation group, single 630n m laser irradiation group, HPPH-PDT group (0.15 mg / kg group, 0.3 mg / kg group, 0.45 mg / kg group) and HpD-PDT 5 mg / kg group. Single injection of HPPH group, HPPH-PDT group and single injection of HPD group, HpD-PDT group were injected photosensitizer from the tail vein, 24 h after irradiation with a wavelength of 665 nm semiconductor laser HPPH-PDT group and a single 665 nm laser group tumor, power At a density of 200 mW / cm2, the energy density was 204 J / cm2 at 17 min for each spot, and the tumor was irradiated with a semiconductor laser at a wavelength of 630 nm in the HpD-PDT group and a single 630 nm laser group with a power density of 200 mW / cm ~ 2, irradiating 20 min per spot, the energy density is 240 J / cm ~ 2. PDT and HpD-PDT were performed at 9 days after PDT in each group (tumor, tumor margin, normal brain tissue), blood mutant type p53 protein (mtp53) and p16 protein expression changes. Results: The expression of mtp53 protein and p16 protein in blank control group, single injection group and control group were all compared with each other, P> 0.05, the difference was not statistically significant. Compared with the blank control group, the expression of mtp53 protein in HPPH-PDT groups and HPD-PDT group were significantly decreased, and the expression of p16 protein was significantly increased, P <0.01, the difference was statistically significant. And close to normal brain tissue value, or p16 protein expression slightly lower than the normal brain value, mtp53 protein expression slightly higher than normal brain tissue. The protein expression of mtp53 decreased and the expression of p16 protein increased in HPPH-PDT group (0.3mg / kg, 0.45mg / kg, 0.15mg / kg HPPH-PDT group and HPD-PDT group, P < The significance of mtp53 expression in HPPH-PDT group (0.45 mg / kg) was slightly lower than that in 0.3 mg / kg HPPH-PDT group, p16 protein expression was slightly higher than 0.3 mg / kgHPPH-PDT group, P> 0.05. The expression of mtp53 in HPPH-PDT 0.3 mg / kg group was lower than that in HpD-PDT 5 mg / kg group, and the expression of p16 protein was higher than that in HpD-PDT 5 mg / kg group, P <0.05. Tumor margin mtp53 protein value slightly higher than the tumor value, the blood is lower than the tumor value; p16 protein value slightly lower edge of the tumor value of the tumor, the blood is higher than the tumor value. Conclusion: HPPH-PDT can reduce the expression of mtp53 protein and increase the expression of p16 protein. Compared with HpD-PDT, HPPH-PDTmtp53 protein expression is lower, p16 protein expression is higher. HPPH 0.3 mg / kg in this experiment is the appropriate HPPH-PDT dose.