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研究了水产品中伊维菌素、阿维菌素、莫能菌素、埃普里诺菌素、多拉菌素的液液萃取-高效液相色谱分析方法。样品用异辛烷进行提取,蒸干后用正己烷溶解,再用乙腈进行反萃取净化,用N-甲基咪唑和三氟乙酸酐进行衍生,经ODS-3柱分离后液相色谱荧光检测器检测。伊维菌素、阿维菌素、莫能菌素、埃普里诺菌素、多拉菌素的的检出限均能达到1.0μg/kg。在添加1～50μg/kg的浓度范围内,平均添加回收率为78.9%～106%,相对标准偏差为5.8%～11.2%。
The liquid-liquid extraction-high performance liquid chromatography (HPLC) method for the determination of ivermectin, abamectin, monensin, epprinomol and doramectin in aquatic products was studied. The sample was extracted with isooctane, evaporated to dryness, dissolved in n-hexane, and then stripped with acetonitrile for purification. The derivatization was carried out with N-methylimidazole and trifluoroacetic anhydride. After separation by ODS-3, the liquid chromatography fluorescence detection Detector. The detection limits of ivermectin, abamectin, monensin, eprinomectin and doramectin all reached 1.0μg / kg. In the range of 1 ~ 50μg / kg, the average recoveries were 78.9% -106%, and the relative standard deviations were 5.8% ~ 11.2%.