琼胶平板稀释纯化法和琼胶平板表面单孢子挑取法是目前我国常用的真菌菌种纯化方法。前者简单易行,但所得菌落难于肯定为单孢形成;后者克服了前者的不足之处,但对于初学者颇花时间。作者在真菌菌种纯化的实践中,将上述两种方法进行综合改进,设计成微块法单孢子分离法。这种方法所需仪器设备简单,结果准确可靠,操作简便易学。作者用此法纯化数种镰刀菌、红麻炭疽病菌、水稻稻瘟病菌等均获得满意结 Agar plate dilution purification and Agar plate surface single spore pick method is currently the common mycoplasma purification methods. The former is simple and easy, but the resulting colonization is difficult to be sure to form a single spore; the latter overcome the deficiencies of the former, but for beginners quite time-consuming. The author in the practice of fungal species purification, the above two methods were comprehensively improved, designed to micro-block method single spore separation. This method requires simple equipment, accurate and reliable results, easy to learn. The author used this method to purify several kinds of Fusarium, anthracnose germplasm, rice blast fungus, etc. were satisfactory knot