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目的:研究高血压糖尿病大鼠心脏肾上腺素受体(AR)的改变与心功能变化之间的关系。方法:采用左肾动脉缩窄和注射链脲佐菌素制高血压糖尿病大鼠模型,放射配体结合实验和离体左心房收缩功能实验等方法观察心脏AR(β-AR和/或α1-AR)及功能的改变。结果:与正常对照相比,高血压糖尿病大鼠心脏β-AR的最大结合容量(Bmax)增加35%(P<0.01),KD值不变;且心脏α1-AR的Bmax也显著增加(P<0.05)。高血压糖尿病大鼠左心房与对照相比,β-AR介导的最大收缩反应(Rmax)下降48%(P<0.01),pD2值不变;α1-AR介导的最大收缩反应也降低41%(P<0.05),pD2值不变。结论:高血压糖尿病大鼠心脏β-AR和/或α1-AR数量代偿性增加,但其介导的最大收缩反应降低,可能与受体后信号转导效应减弱有关。
Objective: To investigate the relationship between cardiac adrenergic receptor (AR) changes and cardiac function in hypertensive diabetic rats. Methods: The left ventricular systolic function (AR) and left ventricular systolic function (AR) were observed in rats with left renal artery narrowing and streptozotocin-induced hypertensive diabetic rats, radioligand binding assay and isolated left atrial systolic function test. AR) and functional changes. Results: Compared with the normal control group, the maximum β-AR binding capacity (Bmax) increased by 35% (P <0.01) and the KD value unchanged in diabetic hypertensive rats; and the Bmax of cardiac α1-AR increased <0.05). Compared with the control group, the maximal contractile response (Rmax) decreased by 48% (P <0.01) and the pD2 value unchanged in the left atrium of hypertensive diabetic rats; the maximal contractile response induced by α1-AR also decreased by 41% % (P <0.05), pD2 unchanged. CONCLUSION: The number of β-AR and / or α1-AR in the heart of hypertensive diabetic rats increases compensatoryly, but the maximal contractile response is decreased, which may be related to the decrease of post-receptor signal transduction.