【摘 要】
：
目的:研究5-烯丙基-7-二氟甲基白杨素(ADFMChR)诱导人肺癌细胞(A549)细胞生长抑制和凋亡的作用.方法:体外培养人肺癌A549细胞,平皿集落形成法测定细胞锚定依赖性生长能力,软
论文部分内容阅读
目的:研究5-烯丙基-7-二氟甲基白杨素(ADFMChR)诱导人肺癌细胞(A549)细胞生长抑制和凋亡的作用.方法:体外培养人肺癌A549细胞,平皿集落形成法测定细胞锚定依赖性生长能力,软琼脂集落形成法测定细胞非锚定依赖性生长能力,流式细胞术(FCM)分析细胞周期和细胞凋亡率;凝胶电泳观察凋亡细胞的基因DNA梯形条带.结果:平皿集落形成法和软琼脂集落形成法结果显示ADFMChR呈剂量依赖性抑制A549细胞生长,FCM分析发现ADFMChR呈剂量依赖性诱导A549细胞凋亡;ADFMChR(10μg/mL)孵育A549细胞48h后,DNA琼脂糖凝胶电泳呈现典型梯形条带.结论:ADFMChR具有抑制人肺癌A549细胞生长和诱导凋亡作用.
其他文献
目的建立简单、快速和灵敏的高效液相色谱串联质谱联用法测定人血浆中克仑特罗的浓度。方法采用Agilent Eclipse XDB-C18色谱柱(4.6 mm×50 mm,1.8μm),流动相为乙腈-0.1%甲
An RF deflecting cavity used for bunch length measurement has been designed and fabricated at Tsinghua University for the Thomson Scattering X-Ray Source.The ca
A batch is a subset of jobs which must be processed jointly in either serial or parallel form. For the single machine, batching, total completion time schedulin
1,25-二羟维生素D3(1,25(OH)2D3)是维生素D3经肝、肾代谢后的主要活性形式,有着重要的生理生化活性[1]。研究发现,1,25(OH)2D3不仅参与钙、磷代谢与骨质钙化,还参与调节细胞
Phase Ⅰ commissioning of the SSRF storage ring on 3.0 GeV beam energy was started at the end of December 2007.A lot of encouraging results have been obtained s
Levels in the neutron-rich 109Ru have been studied by observing the prompt γ-rays following the spontaneous fission fragments of 252Cf. The ground state band a
利用异丁基三乙氧基硅烷作为外给电子体与N-Ⅲ催化剂组成高活性及高氢调敏感性催化体系,在间歇式液相本体法聚丙烯生产工艺中,通过控制每釜氢气加入量为氢气钢瓶压降23～25 MPa
We apply the algebraic Bethe technique to the nuclear pairing problem with certain limits. We obtain the exact energies and eigenstates, and find the symmetry b
In this paper, the author has given an existence theorem for the resonant equa-tion,-△pu=λ1|u|p-2u+f(u)+h(x), without any Landesman-Lazer conditions on h(x).