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目的探讨急性肺损伤（ＡＬＩ）肺组织血小板内皮细胞粘附分子－１（ＰＥＣＡＭ－１）ｍＲＮＡ表达的变化和可能作用。方法在脂多糖（ＬＰＳ）诱发的大鼠ＡＬＩ模型，使用斑点杂交观察肺组织ＰＥＣＡＭ－１ｍＲＮＡ表达的变化。结果正常肺组织表达较高水平的ＰＥＣＡＭ－１ｍＲＮＡ；在ＡＬＩ大鼠肺组织，ＰＥＣＡＭ－１ｍＲＮＡ表达早期下调，但致伤６ｈ时，又基本恢复至正常水平。结论正常肺组织表达较高水平的ＰＥＣＡＭ－１ｍＲＮＡ，ＰＥＣＡＭ－１可能参与ＡＬＩ的发病。
Objective To investigate the changes and possible roles of platelet endothelial cell adhesion molecule-1 (PECAM-1) mRNA expression in lung of acute lung injury (ALI). Methods The changes of PECAM-1mRNA expression in lung tissue were observed by dot blot hybridization in rat LPS-induced ALI model. Results The normal lung tissue expressed higher levels of PECAM-1 mRNA. In the lungs of ALI rats, the expression of PECAM-1 mRNA was down-regulated at the early stage, but returned to normal after 6 hours of injury. Conclusion Normal lung tissue express higher levels of PECAM-1 mRNA, PECAM-1 may be involved in the pathogenesis of ALI.