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目的探讨持续性正加速度(+Gz)暴露后,缺血恢复期大鼠学习能力和海马生长抑素的变化规律。方法雄性SD大鼠80只随机分为对照组、+6Gz/3min组和+10Gz/3min组,观察不同G值+Gz作用后,0d、2d、4d和6d大鼠(每组8只)学习能力的变化,及+Gz暴露后0d、2d和4d大鼠海马生长抑素的变化情况(每时间点每组8只)。结果Y型迷宫测试中,与对照组比较,+6Gz/3min组正确反应次数和反应时在0d无显著性改变,在暴露后2d、4d和6d时正确反应次数均较对照组显著减少(P<0.01),反应时均显著延长(P<0.01);+10Gz/3min组在暴露后各时间点的正确反应次数均显著减少(P<0.01),反应时均显著延长(P<0.01);与+6Gz/3min组比较,+10Gz/3min组的正确数仅在0d时显著减少(P<0.01),反应时显著延长(P<0.05),余无显著差异。放射免疫结果显示,与对照组比较,+6Gz/3min组在暴露后0d,2d海马SS显著降低(P<0.05或P<0.01);+10Gz/3min组在暴露后0d,2d时间点海马SS显著降低(P<0.01)。结论+Gz暴露导致大鼠学习能力受损,海马生长抑素含量降低。
Objective To investigate the changes of learning ability and somatostatin in hippocampus after continuous positive acceleration (+ Gz) exposure. Methods Eighty male Sprague-Dawley rats were randomly divided into control group, + 6Gz / 3min group and + 10Gz / 3min group. After the effects of different G values and Gz were observed, the rats of 0d, 2d, 4d and 6d Ability changes and the changes of somatostatin in hippocampus of rats on day 0, day 2 and day 4 after + Gz exposure (8 rats in each group). Results In Y-maze test, the number of correct reaction in + 6Gz / 3min group and the reaction time did not change significantly at 0d, and the number of correct reaction at 2d, 4d and 6d after exposure was significantly lower than that in control group (P (P <0.01). The number of correct reaction in each group at 10Gz / 3min after exposure was significantly decreased (P <0.01), and the response time was significantly longer (P <0.01). Compared with + 6Gz / 3min group, the correct number in + 10Gz / 3min group was significantly decreased only at 0d (P <0.01), prolonged in response time (P <0.05). The results of radioimmunoassay showed that in + 6Gz / 3min group, the SS of hippocampus was significantly decreased on the 0d and the 2d post-exposure (P <0.05 or P <0.01), while in the + 10Gz / 3min group Significantly lower (P <0.01). Conclusions + Gz exposure leads to impaired learning ability of rats and decreased content of somatostatin in hippocampus.