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为研究两种i Gb3类似物(化学修饰的糖脂4′″-dh-i Gb3和4-HO-i Gb3)对NKT细胞Th1/Th2型细胞因子分泌的影响。采用流式细胞术检测经腹腔注射两种i Gb3类似物后C57BL/6小鼠脾脏NKT细胞数量的变化以及NKT细胞胞内IFN-γ和IL-4的表达水平;用Real-ti me PCR方法检测体外培养的脾脏淋巴细胞与i Gb3类似物共孵育后IFN-γ和IL-4的mRNA表达水平,并用ELISA方法检测孵育上清中IFN-γ和IL-4的含量。结果显示:与i Gb3组相比,经两种i Gb3类似物体内刺激后脾脏NKT细胞的数量都没有显著性变化。糖脂4-dh-i Gb3能够较i Gb3更强地诱导脾脏NKT细胞胞内IFN-γ的表达,也能够上调体外培养的脾脏淋巴细胞IFN-γ的mRNA水平及IFN-γ的分泌,而IL-4在所检测的各个水平上都没有显著性变化。提示化学修饰的糖脂4′″-dh-i Gb3能够诱导C57BL/6鼠脾脏NKT细胞Th1型细胞因子的分泌,而并不显著影响Th2型细胞因子的分泌,从而诱导Th1/Th2型细胞因子平衡向Th1方向偏移。
To investigate the effects of two i Gb3 analogs (chemically modified glycolipid 4 ’“- dh-i Gb3 and 4-HO-i Gb3) on Th1 / Th2 cytokine secretion in NKT cells, flow cytometry After intraperitoneal injection of two i Gb3 analogues, the number of splenic NKT cells in C57BL / 6 mice and the intracellular levels of IFN-γ and IL-4 in NKT cells were detected. Real-ti me PCR was used to detect the spleen lymphocytes The mRNA expression levels of IFN-γ and IL-4 after incubation with i Gb3 analogues were detected by ELISA, and the levels of IFN-γ and IL-4 in the supernatant were detected by ELISA.The results showed that compared with i Gb3 group, There was no significant change in the number of splenic NKT cells stimulated with i Gb3 analogs in vivo. Glycolipid 4-dh-i Gb3 was able to induce stronger intracellular IFN-γ expression in spleen NKT cells than i Gb3, Cultured splenic lymphocytes IFN-γ mRNA levels and IFN-γ secretion, and IL-4 at all levels tested did not change significantly.It suggested that chemically modified glycolipid 4 ’”- dh-i Gb3 can Induced the secretion of Th1-type cytokines in spleen NKT cells of C57BL / 6 mice without significantly affecting the secretion of Th2-type cytokines and inducing T The balance of h1 / Th2 cytokines shifts toward the Th1 direction.