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目的分段克隆SARS冠状病毒PUMC2株的全基因组cDNA。方法以SARS冠状病毒PUMC2株基因组RNA为模板,用RT-PCR扩增cDNA片段,PCR产物经纯化后,连接入pGEM-T载体中,进行序列测定。结果获得了SARS冠状病毒PUMC2株基因组全长cDNA的分段克隆。结论SARS冠状病毒PUMC2株基因组全长cDNA分段克隆的获得,为SARS冠状病毒基因功能的研究和全长有感染性cDNA的克隆奠定了基础。
Objective To clone the genome-wide cDNA of PUMC2 strain of SARS coronavirus. Methods The genomic RNA of PUMC2 strain of SARS coronavirus was used as template to amplify the cDNA fragment by RT-PCR. The PCR product was purified and ligated into pGEM-T vector for sequencing. Results The full length cDNA of PUMC2 strain of SARS coronavirus was cloned. Conclusion The full-length cDNA clone of SARS-CoV PUMC2 strain obtained the foundation for the study of SARS coronavirus gene function and full-length infectious cDNA cloning.