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测定SLE病人外周血白细胞凋亡相关基因Bcl 2和Bcl XmRNA表达量 ,探索凋亡在SLE发病中的作用和意义。采用荧光标记RT PCR半定量方法 ,以β actin为内参照 ,对 5 0例SLE病人、 2 4例RA病人和 2 4例正常人外周血白细胞凋亡相关基因Bcl 2和Bcl XmRNA表达水平进行了检测。结果显示 ,SLE病人Bcl 2表达低于正常人 (1 372 8± 1 2 90 5Vs 1 8418±1 0 15 79,P <0 0 1) ,Bcl Xs表达明显高于正常人 (3 112 6± 2 0 136Vs 1 32 75± 0 6619,P <0 0 0 0 1) ,亦高于RA病人 (3 112 6± 2 0 136Vs 1 30 61± 0 5 30 7,P <0 0 0 0 1) ,Bcl xl无明显改变 ;而RA病人Bcl 2表达低于正常人 (1 0 0 66± 0 5 82 1Vs 1 8418± 1 0 15 79,P <0 0 1) ,Bcl Xs和Bcl xl表达与正常人相比均无统计学差别。SLE病人外周血白细胞抗凋亡的基因表达降低而促进凋亡的基因表达增加 ,提示凋亡在SLE发病中可能具有一定作用和意义。
To determine the expression levels of Bcl-2 and Bcl-XmRNA in peripheral leukocyte apoptosis in patients with SLE and to explore the role and significance of apoptosis in the pathogenesis of SLE. Semi-quantitative RT-PCR was used to detect the expression of Bcl-2 and Bcl-XmRNA in peripheral leukocytes in 50 SLE patients, 24 RA patients and 24 normal controls using β actin as internal reference Detection. The results showed that the expression of Bcl-2 in SLE patients was significantly lower than that in healthy controls (1372 ± 1280Vs 1 8418 ± 1 0 15 79, P <0.01) 0 136Vs 1 32 75 ± 0 6619, P 0 01 0), but also higher than that of RA patients (3 112 6 ± 20 136Vs 1 30 61 ± 0 5 30 7, P 0 01 0) The expression of Bcl-2 and Bcl-2 in RA patients was significantly lower than that in normal controls (10066 ± 0 5 82 1Vs 1 8418 ± 1 0 15 79, P 0 01) No statistically significant difference. SLE patients with peripheral blood leukocyte anti-apoptotic gene expression decreased to promote apoptosis of gene expression, suggesting that apoptosis in the pathogenesis of SLE may have a role and significance.