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目的研究芳烃受体(AHR)基因多态性与焦炉作业工人外周血淋巴细胞染色体损伤易感性的关系。方法选取89名焦炉作业工人(暴露组)和60名无职业性多环芳烃及射线暴露的工人(对照组)为研究对象。测定尿中1-羟基芘反映多环芳烃暴露内剂量,用双核淋巴细胞微核评价个体外周血淋巴细胞染色体损伤,采用聚合酶链反应-限制性片段长度多态性方法分析AHR基因rs6960165及rs2282885位点多态性,利用PHASE2.1软件经Bayesian法计算单体型。用协方差统计方法分析不同基因型及单体型对与染色体损伤水平的关系。结果暴露组尿1-羟基芘水平显著高于对照组(P<0.01),暴露组外周血淋巴细胞微核率显著高于对照组(P<0.01)。在校正年龄和自然对数转换后的1-羟基芘后,暴露组AHR基因rs6960165位点多态性与微核率有关联(P=0.014),未发现对照组AHR基因rs6960165位点多态性与微核率有关联(P=0.586);未发现暴露组和对照组的AHR基因rs2282885位点多态性与微核率有关联(P=0.308和P=0.415)暴露组AHR基因不同单体型对与微核率有关联(P=0.007),未发现对照组AHR基因不同单体型对与微核率有关联(P=0.768)。结论AHR基因rs6960165位点多态性及AHR基因单体型是影响焦炉作业工人外周血淋巴细胞染色体损伤水平易感性的可能因素之一。
Objective To investigate the relationship between the polymorphism of aromatic hydrocarbon receptor (AHR) gene and susceptibility to chromosome damage of peripheral blood lymphocytes in coke oven workers. Methods 89 coke oven workers (exposed group) and 60 non-occupational polycyclic aromatic hydrocarbons (PAHs) and exposed workers (control group) were selected as the study objects. The urinary 1-hydroxypyrene was used to detect the internal dose of polycyclic aromatic hydrocarbons (PAHs) exposure. The peripheral blood lymphocyte chromosome damage was evaluated by binuclear lymphocyte micronucleus, and the rs6960165 and rs2282885 of AHR gene were analyzed by polymerase chain reaction-restriction fragment length polymorphism Site polymorphism, the use of PHASE2.1 software calculated by Bayesian haplotype. The relationship between different genotypes and haplotypes and chromosomal damage was analyzed by covariance statistical method. Results The urinary 1-hydroxypyrene level in exposed group was significantly higher than that in control group (P <0.01). The micronucleus rate of peripheral blood lymphocytes in exposed group was significantly higher than that in control group (P <0.01). After adjusting for age and natural logarithmic conversion of 1-hydroxypyrene, the rs6960165 polymorphism of AHR gene was associated with micronucleus rate (P = 0.014) in the exposed group and no rs6960165 polymorphism of AHR gene was found in the control group (P = 0.586). No association of rs2282885 polymorphism of AHR gene with micronucleus rate was found in exposed group and control group (P = 0.308 and P = 0.415) (P = 0.007). There was no association between different haplotype pairs of AHR gene and micronuclei in the control group (P = 0.768). Conclusion The rs6960165 polymorphism of AHR gene and the haplotype of AHR gene are one of the possible factors that affect the susceptibility of chromosome damage in peripheral blood lymphocytes of coke oven workers.