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目的:观察色胺酮对哮喘豚鼠血清及肺泡灌洗液中NO、NOS浓度的影响。方法:将初筛合格的48只豚鼠随机分成6组:空白组(A组)、慢性模型对照组(B组)、色胺酮高剂量组(C组)、色胺酮中剂量组(D组)、色胺酮低剂量组(E组)和阳性对照组(F组)。采用磷酸卵蛋白(OVA)腹腔注射致敏加雾化吸入激发的方法复制豚鼠哮喘模型,造模成功后给予色胺酮和地塞米松治疗。测定各组豚鼠肺部组织、血清及肺泡灌液(BALF)中NO和NOS浓度并进行组间比较。结果:与B组相比,C、D、E、F组血清中NO和NOS浓度显著降低,组间比较,差异存在统计学意义(P<0.05);各组肺组织中NOS表达呈阴性;C、D、E、F组BALF中NO浓度较B组降低,组间比较,差异存在统计学意义(P<0.05)。结论:色胺酮可降低哮喘豚鼠血清中NO和NOS水平,降低BALF中NO水平,可能对哮喘有一定的防治作用。
Objective: To observe the effects of tryptanthrin on NO and NOS in serum and bronchoalveolar lavage fluid of asthmatic guinea pigs. Methods: Forty-eight guinea pigs were randomly divided into 6 groups: blank group (A group), chronic model control group (B group), high concentration of tryptamine group (C group) Group), low dose of tryptamine group (group E) and positive control group (group F). Guinea pig asthma model was reproduced by intraperitoneal injection of ovalbumin (OVA) and sensitized by inhalation by atomization. After the model was established, trypsin and dexamethasone were given. The concentrations of NO and NOS in lung tissue, serum and alveolar fluid (BALF) of guinea pigs were measured and compared between groups. Results: The concentrations of NO and NOS in group C, D, E and F were significantly lower than those in group B (P <0.05). The expression of NOS in each group was negative. The concentration of NO in BALF in group C, D, E and F was lower than that in group B, and the difference was statistically significant (P <0.05). Conclusions: Secretion of trypsin can reduce the levels of NO and NOS in the serum of asthmatic guinea pigs and decrease the level of NO in BALF, which may have some preventive and therapeutic effects on asthma.