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背景:目前研究认为,Mr 70000热休克蛋白(HSP 70)是缺氧缺血的敏感指标。单唾液酸四已糖神经节苷脂(Monosialotetrahexosylganglioside,GM_1)是哺乳类神经节苷脂的主要种类,HSP70及GM_1在新生儿缺氧缺血性脑损伤(hypoxic-ischemic encephalopathy,HIE)中的作用及机制目前尚不清楚。 目的:研究新生大鼠缺氧缺血性脑损伤(hypoxia-ischemia brain damage,HIBD)后海马CA_1区Mr70000热休克蛋白(HSP70)表达、病理学损伤变化和外源性GM_1对其的影响。 设计:完全随机对照的实验研究。 地点、材料和干预:本实验在解放军第四军医大学航空医学系及第四军医大学西京医院病理科完成。实验采用7日龄SD乳鼠。按随机抽签法分成3组,正常对照组6只,缺氧缺血组24只,缺血后治疗组24只。干预:建立HIBD模型,用免疫组化及苏木素-伊红(HE)染色方法检测缺氧缺血和GM_1干预后不同时间点脑海马组织HSP70阳性细胞及病理学改变。 主要观察指标:正常大鼠、缺氧缺血大鼠及缺氧缺后给予GM_1大鼠的海马CA_1区HSP70染色程度及神经损伤分级比较。 结果:HI后新生大鼠海马CA_1区仅能检出少量阳性HSP核蛋白,呈1级染色,海马CA_1区神经元呈较严重的缺血损伤性改变,损伤程度达3～4级。而GM1组可见应激蛋白HSP 70明显表达,24h达高峰,呈2～3级染色;神
Background: The current study suggests that Mr 70 000 heat shock protein (HSP70) is a sensitive indicator of hypoxia and ischemia. Monosialotetrahexosylganglioside (GM_1) is the main type of mammalian gangliosides, the role of HSP70 and GM_1 in neonatal hypoxic-ischemic encephalopathy (HIE) And the mechanism is not yet clear. Aims: To investigate the expression of heat shock protein 70 (HSP70) in hippocampal CA1 region after hypoxic ischemic brain damage (HIBD) in neonatal rats, the changes of pathological damage and the effect of exogenous GM_1 on it. Design: A fully randomized controlled experimental study. Location, Materials and Interventions: This experiment was completed at the Department of Aeronautical Medicine of the Fourth Military Medical University of PLA and the Xijing Hospital of the Fourth Military Medical University. 7-day-old SD suckling mice were used in the experiment. Divided into 3 groups by random drawing, 6 normal control group, 24 hypoxia-ischemia group and 24 ischemia-reperfusion group. Intervention: HIBD model was established. HSP70 positive cells and pathological changes of hippocampus were detected by immunohistochemistry and hematoxylin-eosin (HE) staining at different time points after hypoxia-ischemia and GM-1 intervention. MAIN OUTCOME MEASURES: HSP70 staining in hippocampal CA1 region of normal rats, hypoxic-ischemic rats and hypoxic-ischemic rats were compared and graded. Results: Only a small amount of positive HSP nuclear protein was detected in hippocampal CA1 region of neonatal rats after HI, showing a grade 1 staining. The neurons in hippocampal CA1 region showed more severe ischemic injury, with a grade of 3-4. In GM1 group, the expression of stress protein HSP70 was observed, reached the peak at 24h, and was stained with grade 2 ~ 3; God