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目的:建立测定杜仲降压片中松脂醇二葡萄糖苷含量的测定方法。方法:色谱柱为DiamonsilC18柱,以乙腈:水(15:85,V/V)作为流动相,流速为0.7ml.min-1,检测波长为227nm。结果:松脂醇二葡萄糖苷在0.05~0.5μg范围内呈良好的线性关系,平均回收率为99.98%,RSD=0.5%(n=6)。结论:建立的方法简便、重现性好、结果准确,可用于松脂醇二葡萄糖苷的测定。
Objective: To establish a method for the determination of pinoresinol diglucoside in Eucommiae antihypertensive tablets. Methods: The column was Diamonsil C18 column with acetonitrile: water (15:85, V / V) as the mobile phase at a flow rate of 0.7ml.min-1 and a detection wavelength of 227nm. Results: Pinoresinol diglucoside showed good linearity in the range of 0.05-0.5 μg with an average recovery of 99.98% and RSD 0.5% (n = 6). Conclusion: The established method is simple, reproducible, accurate and can be used for the determination of pinoresinol diglucoside.