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目的:采用RP-HPLC法测定5～65 d益智果实中圆柚酮和益智酮甲的含量。方法:采用Phenomenex Gemini C6-phen-yl色谱柱(250 mm×4.6 mm,5μm),以乙腈(A)-水(B)为流动相,梯度洗脱[0～15 min,A-B(30∶70);16～50 min,A-B(30∶70)→A-B(44∶56);51～60 min,A-B(44∶56);61～65 min,A-B(44∶56)→A-B(70∶30)],流速1.0 mL.min-1,检测波长为240 nm。结果:圆柚酮和益智酮甲的线性范围分别为0.06～2.03μg(r=0.9999)和0.004～1.14μg(r=0.9995),加样回收率(n=6)分别为97.53%%和98.31%。益智中的成分在55 d后,含量趋于稳定。结论:本文方法可以为益智的采收时间提供科学参考。
OBJECTIVE: To determine the content of mevalonate and mechlorethamine in Fructus Puchi fruits from 5 to 65 d by RP-HPLC. METHODS: The mobile phase consisted of Phenomenex Gemini C6-phen-yl column (250 mm × 4.6 mm, 5 μm) and acetonitrile (A) -water (B) ); AB (44:56); AB (44:56) → AB (70:30); AB )], The flow rate was 1.0 mL.min-1, the detection wavelength was 240 nm. Results: The linear ranges of pomelozone and yogurt ketone were 0.06 ~ 2.03μg (r = 0.9999) and 0.004 ~ 1.14μg (r = 0.9995) respectively, and the recovery rates were 97.53% and 98.31%. The content of puzzle in the 55 d after the content stabilized. Conclusion: This method can provide a scientific reference for the recovery time of puzzle.