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B类MADS-box基因在调控显花植物的花瓣和雄蕊发育过程中发挥关键作用。为初步了解大蒜花发育的分子机制,以‘阿城紫皮’大蒜(Allium sativum)的花蕾为试材克隆了AsPI(Gen Bank登录号为KY272748)。AsPI的cDNA长939 bp,开放阅读框为615 bp,编码204个氨基酸。推导的氨基酸序列显示,AsPI编码的蛋白质包含高度保守的MADS结构域(1~57氨基酸)和保守的K结构(76~140氨基酸),AsPI蛋白具有PI亚家族特有的序列特征:MADS区特征丝氨酸残基、K区高度保守序列KHExL以及C–末端的PI基序。序列比对、功能域分析及系统发育分析结果表明,AsPI属于单子叶植物B功能基因PI亚家族。半定量和实时RT-PCR检测AsPI在大蒜不同组织器官中的表达模式,结果表明,在花蕾中高丰度表达,在花茎中表达量中等,在根、假茎、嫩叶、保护叶等营养器官中表达量极低甚至不表达。
The class B MADS-box gene plays a key role in the regulation of petal and stamen development in flowering plants. To understand the molecular mechanism of garlic flower development, AsPI (Gen Bank accession number KY272748) was cloned from the buds of Allium sativum. AsPI cDNA has a length of 939 bp and an open reading frame of 615 bp, encoding 204 amino acids. The deduced amino acid sequence showed that the protein encoded by AsPI contains a highly conserved MADS domain (1-57 amino acids) and a conserved K structure (76-140 amino acids). AsPI protein has the characteristic sequence of the PI subfamily: the characteristic serine Residues, KHExL, a highly conserved sequence in the K region, and the PI motif at the C-terminus. Sequence alignment, functional domain analysis and phylogenetic analysis indicated that AsPI belonged to the PI subfamily of functional B genes in monocots. Semi-quantitative and real-time RT-PCR was used to detect the expression patterns of AsPI in different tissues and organs of garlic. The results showed that the expression pattern of AsPI was highly abundant in flower buds and moderately expressed in flower stems. Vegetative organs such as root, pseudostem, In the expression of very low or not expressed.