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乙型肝炎病毒(HBV)所致的肝细胞坏死,被认为是与人类白细胞抗原(HLA)有关的MHC限制的T细胞为主的免疫应答的结果。本研究证实,转染HBV基因组或X基因后的人肝癌细胞系可诱导HLAⅡ(即HLA DR)表达。核转录及RNA杂交分析提示表达X基因的细胞其HLA DR mRNA水平增加,X蛋白增加该基因的转录。氯霉索乙酰基转移酶(CAT)基因分析进一步提示,X蛋白通过作用于HLA DR基因上游的调节序列诱导HLA DR表达。结果表明:X蛋白可能通过调节HLA DR表达参与HBV感染的免疫发病机理。
Hepatocyte necrosis due to Hepatitis B virus (HBV) is considered as a result of an MHC-restricted T cell-based immune response associated with human leukocyte antigen (HLA). This study confirmed that human hepatocellular carcinoma cell line transfected with HBV genome or X gene can induce HLA ¢ ò (HLA DR) expression. Nuclear transcription and RNA hybridization analysis revealed that the cells expressing the X gene increased their HLA DR mRNA levels and X protein increased the transcription of the gene. Analysis of the chloramphenicol acetyltransferase (CAT) gene further suggests that the X protein induces HLA DR expression by acting on regulatory sequences upstream of the HLA DR gene. The results showed that X protein might participate in the immune pathogenesis of HBV infection by regulating HLA DR expression.