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目的 :探讨一氧化氮 (NO)及其合酶 (NOS)在鼻息肉中的表达 ,以及NO在鼻息肉发病中的作用。方法 :用免疫组织化学及原位杂交方法研究诱导型一氧化氮合酶 (iNOS)及内皮型一氧化氮合酶 (eNOS)在鼻息肉中的表达 ,同时用原位杂交方法研究iNOSmRNA的表达 ,并用硝酸还原酶法研究NO在鼻息肉中的产生情况。结果 :鼻息肉组织中eNOS主要分布于上皮、腺体细胞及血管内皮细胞 ,其染色强度稍强于对照组。iNOS在上皮细胞呈现较强的阳性染色 ,在息肉组织内主要表达于散在的炎症细胞。结论 :eNOS活性增高可能与鼻息肉发病中血管过度扩张、腺体病理性分泌增多等有关。iNOS生成的较高浓度的NO在鼻息肉的病理过程中可能起到促进炎症发展的作用。
Objective: To investigate the expression of nitric oxide (NO) and its synthase (NOS) in nasal polyps and the role of NO in the pathogenesis of nasal polyps. Methods: The expressions of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in nasal polyps were studied by immunohistochemistry and in situ hybridization. The expression of iNOS mRNA was detected by in situ hybridization , And nitric acid reductase was used to study the production of NO in nasal polyps. Results: The eNOS in nasal polyps was mainly distributed in the epithelium, glandular cells and vascular endothelial cells, and its staining intensity was slightly stronger than that of the control group. iNOS in epithelial cells showed a strong positive staining in polyp tissue mainly expressed in scattered inflammatory cells. Conclusion: Increased eNOS activity may be related to over-expansion of blood vessels and increased secretion of pathological glands in nasal polyps. The higher concentration of NO produced by iNOS may play an important role in promoting the development of inflammation during the pathological process of nasal polyps.