丹参分支酸变位酶基因的克隆和生物信息学分析

来源 :中国中药杂志 | 被引量 : 0次 | 上传用户:zbbankcomm
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
应用BlastX检索丹参毛状根的EST数据库,发现1条与分支酸变位酶(chorismate mutase,CM)高度同源的序列,克隆得到其全长cDNA,命名为SmCM1,Genbank登录号为KC784342。SmCM1全长948 bp,理论pI 6.41,与矮牵牛Petunia×hybrida、拟南芥Arabidopsis thaliana和毛果杨Populus trichocarpa的CM1分别具有70%,72%,64%的相似性。实时荧光定量PCR(real time quantitative PCR,QRT-PCR)分析表明SmCM1在丹参叶中的含量较高,其次是茎,在根中的含量相对较低。酵母诱导子(YE)和离子(Ag+)联合诱导丹参毛状根后,SmCM1与莽草酸途径的关键酶3-脱氧-D-阿拉伯-庚酮糖-7-磷酸合酶(3-deoxy-7-phosphoheptulonate synthase,DAHPS)和分支酸合酶(chorismate synthase,CS)基因表达趋势呈协同变化。YE+Ag+处理后3个基因皆上调表达,8 h达到表达高峰,分别是对照的7.9,5.5,9.8倍,随后下调,36 h时下降至对照水平以下,说明诱导处理之后糖代谢中间产物经莽草酸和分支酸途径合成苯丙氨酸和酪氨酸从而引起酚酸类化合物的过量积累。 BlastX was used to search the EST database of hairy root of Salvia miltiorrhiza and one highly homologous to chorismate mutase (CM) was found. The full-length cDNA was cloned and named as SmCM1. Genbank Accession No. KC784342. SmCM1 is 948 bp in length and has a theoretical pI of 6.41. It has 70%, 72% and 64% similarity to CM1 of petunia × hybrida, Arabidopsis thaliana and Populus trichocarpa, respectively. Real-time quantitative PCR (QRT-PCR) analysis showed that the content of SmCM1 in Salvia miltiorrhiza leaves was higher, followed by stems, and its content in roots was relatively lower. Induction of the hairy roots of Salvia miltiorrhiza by the combination of yeast elicitor (YE) and ion (Ag +) led to the interaction between SmCM1 and 3-deoxy-7, a key enzyme in the shikimic acid pathway -phosphoheptulonate synthase (DAHPS) and chorismate synthase (CS) genes showed synergistic changes. After YE + Ag + treatment, the three genes were up-regulated and peaked at 8 h, which were respectively 7.9, 5.5 and 9.8 times of the control, then down-regulated and below 36 h after the treatment, indicating that after induction treatment, the glucose metabolism intermediate product Shikimate and chorismate pathways synthesize phenylalanine and tyrosine to cause excessive accumulation of phenolic acids.
其他文献
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生、测量监控等方面人手,介绍了S226海滨大桥
期刊
铁路在中国近代历史上具有重要意义,而清末民初的铁路国有政策作为引发辛亥革命的直接原因更值得关注。铁路国有政策要解决什么样的问题,它是怎样产生出来的,为什么它会引起革命
民族主义是一个发展的历史概念,是现代化过程的产物,是民族意识和爱国主义的融合,其最终目标就是建立民族国家并实现国家的现代化。民族主义的最高境界是爱国主义,爱国主义是其核
请下载后查看,本文暂不支持在线获取查看简介。 Please download to view, this article does not support online access to view profile.
期刊
近代的日本从明治维新后,逐渐走上了发展资本主义的道路。各项改革措施使日本在短时期内,在政治、经济、军事、社会生活等方面发生了巨大的变化。实力增强的日本开始对外实施扩
在十九世纪形成于德国的以兰克为代表的实证主义史学是受当时自然科学的影响并且继承了以往历史学家在历史写作中注重史料批判的优良传统。当时的德国各个知名大学则为这两种
中国近代邮政事业在北京政府时期获得快速发展,尤其在邮权的统一、邮政营业规模的扩大以及邮政设施的完善等方面取得了良好成绩。 本文着重从管理制度、业务经营、西方影响
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生、测量监控等方面人手,介绍了S226海滨大桥
期刊
本文对雍乾嘉查禁天主教教案进行了法制考察。文章认为,雍乾嘉查禁天主教具有四个特点:一、总的趋势是查禁越来越严厉,法律条文越来越明确和严密;二、雍乾嘉查禁天主教受皇帝个人