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目的:观察强精煎对实验小鼠睾丸Fas、Fasl、Bcl-2和Bax蛋白表达的影响。方法:将80只健康雄性昆明种小鼠分为正常组、模型组、强精煎组、黄精赞育胶囊组,以环磷酰胺注射液40mg/kg腹腔注射制造小鼠生精功能障碍模型,以HE染色观察小鼠睾丸病理形态,采用Johnson评分评价精子发生和发生障碍程度,用免疫组织化学SABC法检测Fas、Fasl、Bcl-2和Bax蛋白在小鼠睾丸实质组织中的表达率。结果:模型组小鼠睾丸组织可见明显的病理损伤,强精煎组则生精功能良好,黄精赞育胶囊组稍差。与正常组比较,模型组Johnson评分偏低,且Fas和Fasl蛋白表达率偏高(P<0.01),强精煎组和黄精赞育胶囊组与正常组各方面差异均无统计学意义(P>0.05)。与模型组比较,正常组、强精煎组和黄精赞育胶囊组睾丸Johnson评分偏高,Fas、Fasl和Bax蛋白表达率显著降低(P<0.01),Bcl-2表达率显著升高(P<0.01),细胞内Bcl-2/Bax比值显著升高(P<0.01)。结论:强精煎可以抑制Bax、Fas和Fasl,并促进Bcl-2蛋白在受损伤小鼠睾丸实质组织中的表达。
Objective: To observe the effect of QJ on protein expression of Fas, Fasl, Bcl-2 and Bax in testes of experimental mice. Methods: Eighty healthy male Kunming mice were divided into normal group, model group, Qiangjing decoction group and Huangjingzanyu capsule group. The mouse model of dysfunctional spermatogenesis was induced by intraperitoneal injection of cyclophosphamide 40mg / kg, The histopathology of the testis was observed by HE staining. The spermatogenesis and degree of dysplasia were evaluated by Johnson score. The expression of Fas, Fasl, Bcl-2 and Bax protein in testicular parenchyma of mice was detected by immunohistochemical SABC method. Results: The pathological changes of testis were observed in the model group, the function of spermatogenesis was better in the strong Jing group, and the slightly lower in the Huang Jing Zang Yu capsule group. Compared with the normal group, the Johnson score of the model group was lower, and the expression rates of Fas and Fasl protein were higher (P <0.01), and there was no significant difference between the strong Jingjian group and the Huangjingzanyu capsule group and the normal group (P > 0.05). Compared with the model group, the scores of Johnson’s testosterone, Fas, Fasl and Bax in the normal group, the strong Jingjian group and the Huangjingzanyu capsule group were significantly lower (P <0.01), and the expression of Bcl-2 was significantly increased <0.01). The ratio of Bcl-2 / Bax in cells was significantly increased (P <0.01). Conclusion: Jianjing decoction can inhibit Bax, Fas and Fasl, and promote the expression of Bcl-2 protein in the damaged testis parenchyma.