目的　研究迟缓爱德华菌 (EdwardsiellatardaEt.)重要毒力因子的溶血素。方法　用鸟枪法将Et- 12株的染色体经Sau3A酶切后 ,连接在质粒pACYC184上。结果　在抗性绵羊红细胞平板上 ,筛选出 7株溶血相关克隆子 ,其中 2株能稳定表达 ,并将 1株重组质粒经酶切后 ,发现其酶谱和大小和国外报告的不同。经斑点杂交 ,证实该溶血相关基因在Et- 12染色体上。结论　可能克隆到新的Et- 12溶血相关基因 Objective To study the hemolysin, an important virulence factor of Edwardsiellaarda Et. Methods The chromosome of Et-12 was digested with Sau3A using shotgun method and ligated into plasmid pACYC184. Results Seven hemolysis-related clones were screened on resistant red sheep plate. Two of them were stably expressed, and one of the recombinant plasmids was digested and found to be different in enzyme profile and size from those reported abroad. Speckle hybridization confirmed that the hemolysis related genes were on the Et 12 chromosome. Conclusion It is possible to clone a new Et-12 hemolysis-related gene