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目的探讨肿瘤坏死因子α(TNFα)及β(TNFβ)基因多态性与多发性硬化(MS)的相关性。方法采用序列特异性引物和聚合酶链式反应(PCR)、限制性内切酶酶切技术对60例MS患者(MS组)和110例健康者(对照组)的TNFα及β基因多态性进行分析。结果MS组TNFα-308等位基因分布频率与对照组比较有显著性差异(P=0．04),且MS组等位基因A含量(3．3%)与对照组(11．8%)比较也有显著性差异(P=0．008)；MS组TNFβ+252等位基因分布频率与对照组比较有显著性差异(P=0．014),且MS组等位基因A含量(69．2%)与对照组(55．5%)比较也有显著性差异(P=0．014)。结论TNFα-308 A等位基因的多态性与MS的发病有一定负相关,TNFβ+252 A等位基因频率及TNFβ+252 A/A基因分布频率与MS的发病有一定正相关。
Objective To investigate the relationship between polymorphisms of tumor necrosis factor α (TNFα) and β (TNFβ) gene and multiple sclerosis (MS). Methods Sequence-specific primers, polymerase chain reaction (PCR) and restriction endonuclease digestion were used to detect the polymorphisms of TNFα and β gene in 60 MS patients (MS group) and 110 healthy controls (control group) Analyze. Results The frequency of TNFα-308 allele distribution in MS group was significantly different from that in control group (P = 0.04). The allele A level in MS group (3.3%) was significantly higher than that in control group (11.8% (P = 0.008). The distribution frequency of TNFβ + 252 allele in MS group was significantly different from that in control group (P = 0.014), and the content of allele A in MS group was significantly higher than that in control group (P = 0.008). 2%) compared with the control group (55.5%) also had significant difference (P = 0.014). Conclusions The polymorphism of TNFα-308 A allele is negatively correlated with the onset of MS. The frequency of TNFβ + 252 A allele and the distribution of TNFβ + 252 A / A gene are positively correlated with the onset of MS.