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利用PCR技术对从日本引进的Tos17插入水稻极限糊精酶基因变异体后代进行了插入位点鉴定和纯合体筛选分析。结果表明,利用Tos17末端2 6bpDNA序列和插入位点上下游各2 0 0bp和30 0bp处的目标基因序列片段设计引物,可以有效地进行Tos17插入突变纯合体筛选,为突变体的进一步利用提供了分子依据。
PCR-based technique was used to identify insertion sites and homozygote selection of Tos17 introduced into Japan by introducing the limit mutant of rice dextrinase. The results showed that the Tos17 insertion 2hour endostatin 2 6bpDNA sequence and the 200 bp and 300 bp upstream and downstream of the insertion site were used to design the primers for Tos17 insertion mutation homozygotes, which provided the further utilization of mutants Molecular basis.