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应用聚合酶链反应-单链构象多态性(Polymerase Chain reaction-single strand conf ORmation polym ORphic,PCR-SSCP)结合直接测序的方法,检测289例山东沿海地区汉族无亲缘关系的个体SIRT1的分布情况。研究对象共分为三组:正常对照组(N组)146例,单纯2型糖尿病组(DM组)66例,2型糖尿病微量蛋白尿组(DN组)77例,对各组等位基因频率/基因型频率进行比较分析结果:糖尿病组的等位基因G与糖尿病的发病风险降低有关,频率明显低于对照组,在糖尿病患者中,GG基因型在糖尿病微量蛋白尿组患者的分布明显多于单纯糖尿病患者组,糖尿病微量蛋白尿组的等位基因G频率明显高于单纯糖尿病组,以等位基因A为对照,等位基因G也与糖尿病微量蛋白尿组的发病风险增高有关。结论:SIRT1-rs2236319基因多态性的等位基因G与2型糖尿病的发病风险降低有关,rs2236319基因多态性的GG基因型和等位基因G与2型糖尿病微量蛋白尿组的发病风险增高有关,血糖、血脂代谢紊乱是2型糖尿病早期肾病发病的危险因素。
The distribution of SIRT1 in 289 Han unrelated individuals in Shandong coastal area was detected by polymerase chain reaction-single strand conformation polym OR (PCR-SSCP) and direct sequencing. . The subjects were divided into three groups: 146 cases in normal control group (N group), 66 cases in DM group (DM group) and 77 cases in DN group (DN group) Frequency / genotype frequency of the results of the analysis: the diabetic group allele G and diabetes risk reduction, the frequency was significantly lower than the control group, in patients with diabetes, GG genotype distribution in patients with diabetic microalbuminuria significantly Compared with simple diabetic group, the frequency of allele G in diabetic microalbuminuria group was significantly higher than that in simple diabetic group. Allele A was the control, and allele G was also associated with increased risk of developing diabetic microalbuminuria. CONCLUSIONS: The allele G of SIRT1-rs2236319 polymorphism is associated with a decreased risk of type 2 diabetes. The genotypes GG and G of rs2236319 polymorphism are associated with an increased risk of developing type 2 diabetes mellitus Related, blood glucose, dyslipidemia is a risk factor for type 2 diabetes early nephropathy.