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采用梯度法、正交试验L9(34)研究盐度、温度、光照强度、光照周期和氮水平对青岛大扁藻、亚心形扁藻、微绿球藻脂肪酸组成的影响。结果指出适宜于合成n-3PUFA的培养条件分别为青岛大扁藻20℃、1000lx、12∶12(LH∶DH)、2份氮水平(2×74.8mg/LNaNO3)、盐度5;亚心形扁藻为20℃;微绿球藻为25℃、4000lx、12∶12(LH∶DH)及2份氮水平。如果作为生物饵料,两种扁藻提供的必需脂肪酸主要是18∶3n-3,微绿球藻主要是20∶5n-3。
Gradient method and orthogonal experiment L9 (34) were used to study the effects of salinity, temperature, light intensity, light period and nitrogen level on the fatty acid composition of P. maculata, The results showed that the culture conditions suitable for the synthesis of n-3PUFA were 20 ℃, 1000lx, 12:12 (LH: DH), 2 nitrogen levels (2 × 74.8mg / LNaNO3) Platymonas subcordiformis was 20 ° C; Microcysculina was 25 ° C, 4000 lx, 12:12 (LH: DH) and 2 parts nitrogen level. If used as a biological bait, the two essential algae provide essential fatty acids predominately 18: 3 n-3, and Microcystis globulus predominately 20: 5 n-3.