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目的:建立和肝利胆颗粒中栀子苷和黄芩苷的含量测定方法。方法:采用高效液相色谱法,用Dionex C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇为流动相A,0.5%冰醋酸溶液为流动相B,梯度洗脱,流速为1.0 ml·min-1,检测波长为239nm,柱温:30℃,进样量:10μl。结果:栀子苷和黄芩苷与其相邻杂质峰能完全分离,栀子苷在7.890~78.900μg·ml-1的浓度范围内有良好的线性关系,r=0.999 9;黄芩苷在8.020~80.200μg·ml-1的浓度范围内有良好的线性关系,r=0.999 9。栀子苷和黄芩苷的平均回收率分别为99.8%(RSD=2.1%,n=6)和99.5%(RSD=1.5%,n=6)。结论:本方法简便、准确、重复性好,可用于该药的质量控制的评价。
Objective: To establish a method for the determination of geniposide and baicalin in Hegan Lidan Granules. Methods: High performance liquid chromatography (HPLC) was performed on a Dionex C18 (250 mm × 4.6 mm, 5 μm) column using methanol as mobile phase A and 0.5% glacial acetic acid as mobile phase B with gradient elution at a flow rate of 1.0 ml · min-1, detection wavelength of 239nm, column temperature: 30 ℃, injection volume: 10μl. Results: Geniposide and baicalin were completely separated from the adjacent impurity peaks. Geniposide had a good linear relationship in the concentration range of 7.890 ~ 78.900μg · ml-1, r = 0.999 9; baicalin was between 8.020 ~ 80.200 There was a good linear relationship between μg · ml-1 and r = 0.999 9. The average recoveries of geniposide and baicalin were 99.8% (RSD = 2.1%, n = 6) and 99.5% (RSD = 1.5%, n = 6), respectively. Conclusion: The method is simple, accurate and reproducible, and can be used to evaluate the quality control of the drug.