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目的:利用HPLC同时测定菊花中绿原酸、1,5-二咖啡酰奎宁酸、异绿原酸A、异绿原酸C、木犀草素-7-O-β-D-葡萄糖苷和芹菜素-7-O-β-D-葡萄糖苷含量。方法:以Phenomenex Gemini-NX C18色谱柱(4.6 mm×250 mm,5μm),甲醇-0.4%磷酸水溶液梯度洗脱,流速1 mL.min-1,柱温25℃,检测波长350 nm。结果:6种活性成分均达到基线分离,线性关系良好(r≥0.999 7);平均加样回收率为100.6%～102.4%,RSD<3%。除异绿原酸A外,其他5种成分在蒸制样品中均显著高于直接晒干样品。蒸制后,半开放菊花绿原酸和异绿原酸A含量分别比全开放菊花高53%和41%。结论:本方法方法灵敏、准确、可靠、重复性好,可用于菊花药材的质量评价。
OBJECTIVE: To determine the contents of chlorogenic acid, 1,5-dicaffeoylquinic acid, iso-chlorogenic acid A, iso-chlorogenic acid C, luteolin-7-O-β-D-glucoside and Apigenin -7-O-β-D-glucoside content. METHODS: The gradient elution was performed on a Phenomenex Gemini-NX C18 column (4.6 mm × 250 mm, 5 μm) with a gradient of methanol-0.4% phosphoric acid. The flow rate was 1 mL.min-1 and the column temperature was 25 ℃. The detection wavelength was 350 nm. Results: All the six active ingredients achieved baseline separation with good linearity (r ≥ 0.997). The average recoveries were 100.6% -102.4% with RSD <3%. In addition to iso-chlorogenic acid A, the other five components in steamed samples were significantly higher than the direct dried samples. After steaming, the contents of chlorogenic acid and iso-chlorogenic acid in semi-open chrysanthemum were 53% and 41% higher than those in open chrysanthemum respectively. Conclusion: The method is sensitive, accurate, reliable, reproducible and can be used for quality evaluation of medicinal materials of chrysanthemum.