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聚合酶链式反应及单链构象多态性技术作为一种区分检测基因组之间微小差异的有效方法 ,具有快速、简便、灵敏和适用于大样品量筛选的特点 .本文详细介绍了该技术的条件优化及改进策略 .该技术在植物病毒学上主要应用于 :(1)分子变异 ;(2 )混合侵染的检测 ;(3)分子流行病学等方面
Polymerase chain reaction and single-strand conformation polymorphism, as an effective method for distinguishing small differences between genomes, is characterized as fast, simple, sensitive and suitable for large sample size screening.In this paper, Condition optimization and improvement strategy.This technology is mainly applied in plant virology: (1) molecular variation; (2) mixed infection detection; (3) molecular epidemiology and other aspects