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通过酶活性测定和高效液相色谱分析,研究经病原菌诱导后黄瓜叶片中脂氧合酶(LOX)活性与茉莉酸(JA)积累量的关系。结果表明,黄瓜经褐斑菌诱导后,叶片中茉莉酸(JA)大量积累,同时脂氧合酶(LOX)活性上升,接种叶片的上位叶中LOX活性和JA含量于诱导接种褐斑菌12h后开始升高,并于诱导接种24h后达到峰值,接种叶片的下位叶中LOX活性和JA含量在诱导接种12h后变化不大,但在24h后显著升高,并于48h达到峰值,此后逐渐平稳下降。相关分析表明,LOX与JA呈显著正相关,相关系数r为0.92。同时通过喷施脂氧合酶抑制剂(NDGA)和添加茉莉酸的试验证实LOX是JA合成的关键酶。
The relationship between the activity of lipoxygenase (LOX) and the accumulation of jasmonic acid (JA) in cucumber leaves induced by pathogen was studied by enzyme activity assay and high performance liquid chromatography (HPLC). The results showed that the accumulation of jasmonic acid (JA) in leaf and the increase of lipoxygenase (LOX) activity in cucumber induced by P. melitensis showed that LOX activity and JA content in the upper leaves of inoculated leaves were significantly higher than that of the control And began to increase after 24 hours after inoculation. The LOX activity and JA content in the lower leaves of inoculated leaves did not change much at 12h after inoculation, but increased significantly after 24h and peaked at 48h, then gradually increased Steady decline. Correlation analysis showed that there was a significant positive correlation between LOX and JA, and the correlation coefficient r was 0.92. At the same time by spraying lipoxygenase inhibitor (NDGA) and adding jasmonic acid test LOX is JA synthesis key enzyme.