目前尚无一种适于在石蜡切片上同时明确地显示肥大细胞与其它结缔组织细胞的染色方法。本研究创造一种以甲基绿、天青Ⅱ、亚甲基蓝,曙红Y配制成的染液,可同时明确地显示肥大细胞与其它结缔组织细胞。本研究探讨了不同pH的本染液对结缔组织肥大细胞与粘膜肥大细胞染色效果的影响,求得最佳染色效果的染液pH值及宜用固定液的种类。并对过去的各种常用肥大细胞染色法与本法作了比较观察。 At present, there is no method suitable for staining both mast cells and other connective tissue cells in paraffin sections. In this study, a dye solution prepared with Methyl Green, Azure II, Methylene Blue, and Eosin Y was used to clearly show both mast cells and other connective tissue cells. This study explored the different pH of the dye solution on the connective tissue mast cells and mucosal mast cells staining effect, to find the best staining effect of the dye pH value and the appropriate fixative species. And the past, a variety of commonly used mast cell staining and the law made a comparative observation.