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目的:探讨Neuropilin-1阳性T细胞(Nrp-1+T细胞)在肿瘤免疫中的调节作用。方法:通过建立B16-F10-luc-G5黑色素瘤细胞体内模型及利用活体萤光成像系统,将由小鼠脾脏细胞中分选出的Nrp-1+T细胞注射入模型小鼠体内,观察Nrp-1+T细胞对黑色素瘤细胞体内模型增殖的影响。结果:Nrp-1+T细胞占脾脏淋巴细胞的比例为(2.65±0.28)%,肿瘤模型建立6d后,Nrp-1+T细胞干预组肿瘤检测光子数高于非Nrp-1+T细胞干预组〔(3.69±0.04)×106s-1vs(1.22±0.09)×105s-1〕,两者差异有统计学意义,t=-43.438,P<0.01。结论:Nrp-1+T细胞在体内能够负性调节针对黑色素瘤细胞的免疫反应,作用机制可能与其抑制NK细胞或直接促进肿瘤细胞增殖有关,Nrp-1+T细胞可能是一种新的Treg亚群。
Objective: To investigate the role of Neuropilin-1 positive T cells (Nrp-1 + T cells) in tumor immunity. Methods: The Nrp-1 + T cells isolated from mouse spleen cells were injected into the model mice by establishing the in vivo model of B16-F10-luc-G5 melanoma cells and in vivo fluorescent imaging system. The Nrp- 1 + T cells on proliferation of melanoma cells in vivo. Results: The ratio of Nrp-1 + T cells to splenic lymphocytes was (2.65 ± 0.28)%. After the establishment of tumor model for 6 days, the number of photon of tumor detection in Nrp-1 + T cells intervention group was higher than that of non-Nrp-1 + T cells Group 〔(3.69 ± 0.04) × 106s-1vs (1.22 ± 0.09) × 105s-1〕, the difference was statistically significant, t = -43.438, P <0.01. CONCLUSION: Nrp-1 + T cells can negatively regulate the immune response against melanoma cells in vivo. The mechanism may be related to the inhibition of NK cells or directly promote the proliferation of tumor cells. Nrp-1 + T cells may be a new Treg Subgroups.