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目的:探讨脑出血(ICH)患者载脂蛋白E(ApoE)和血管紧张素Ⅱ受体-1(AT1R)A1166C基因多态性是否有协同致ICH效应。方法:应用聚合酶链反应-限制性片段长度多态性技术检测80例ICH患者和90名健康对照者ApoE和AT1R A1166C基因多态性,并运用logistic回归分析ApoE和AT1R A1166C基因多态性致ICH的效应。结果:ICH组ApoE基因型频率和等位基因频率与正常对照组无显著差异;AT1R AC基因型频率为32.5%,C等位基因频率为16.2%,显著高于正常对照组的11.1%和5.6%(P均<0.05);logistic回归分析显示,AT1R C等位基因个体发生ICH的优势比(OR)为3.299(95%CI 1.537～7.079,P<0.05);同时携带ATIR C等位基因和ApoEε4等位基闪的个体患ICH的OR为4.784(95%CI 1.325～17.272,P<0.05)。结论:AT1R A1166C基因多态性可能是ICH发病的独立遗传因素;AT1R A1166C基因多态性与ApoE基因多态性具有协同致ICH效应。
Objective: To investigate whether there is synergistic ICH effect in A1166C polymorphism of ApoE and AT1R in patients with intracerebral hemorrhage (ICH). Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect ApoE and AT1R A1166C gene polymorphisms in 80 ICH patients and 90 healthy controls. Logistic regression was used to analyze the polymorphisms of ApoE and AT1R A1166C Effect of ICH. Results: The frequencies of ApoE genotype and allele in ICH group were not significantly different from those in normal control group. The frequency of AT1R AC genotype was 32.5% and the frequency of C allele was 16.2%, significantly higher than that of 11.1% and 5.6% % (All P <0.05). Logistic regression analysis showed that the odds ratio (OR) of ICH for AT1R C allele was 3.299 (95% CI 1.537-7.079, P <0.05) Individuals with ApoE4 alleles had ICH of 4.784 (95% CI 1.325-17.272, P <0.05). CONCLUSION: AT1R A1166C gene polymorphism may be an independent genetic factor in the pathogenesis of ICH. The AT1R A1166C gene polymorphism has a synergistic ICH effect with ApoE gene polymorphism.