目的：分析蟾饲五谷虫有效成分并研究其免疫活性。方法：采用薄层色谱扫描法及体内免疫活性成分测定方法。结果：蟾词五谷虫可显著降低小鼠胸腺及脾脏系数，降低血清IgG含量，并显著抑制机体淋巴细胞转化率。结论：蟾词五谷虫对机体免疫活性的抑制可能源于其主要有效成分华蟾酥毒基等。 Objective: To analyze the active ingredients of pupa-feeding pupa and study its immunological activity. Methods: Thin-layer chromatography and in vivo immunoassay methods were used. RESULTS: The eucalyptus granuloma significantly reduced the thymus and spleen coefficient, reduced the serum IgG level, and significantly inhibited the lymphocyte transformation rate. Conclusion: The inhibitory effect of the eucalyptus granuloma on the immune activity of the eucalyptus may be attributed to its main active ingredient, such as Huaxuanxuan.