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利用室内叶片快速鉴定法结合田间抗性调查 ,研究了粳型 Bt转基因水稻“克螟稻”与籼、粳稻杂交后代对二化螟 ( Chilo suppressalis)的抗性。结果显示 ,在与籼稻和粳稻的杂交后代 ( F1、F2 和 BC1)中 ,都出现了对二化螟表现高抗的植株。用离体叶片法测定 ,4个组合 12 0株杂种 F1和 88株 GUS阳性( GUS+) BC1株与 30株克螟稻亲本相似 ,2天内对二化螟初孵幼虫的死亡率高达 10 0 .0 % ,而用相应组合的 GUS阴性 ( GUS-)株和非转基因亲本叶片饲喂的二化螟初孵幼虫 4天内的死亡率仅为 8.33%～ 2 2 .8%。此外 ,在未施用对鳞翅目害虫有毒性的农药的试验田 ,共调查 8个组合 70 7株 GUS+F2 植株和 7个组合 177株 GUS+BC1植株 ,仅有大约 1%的植株出现枯心和白穗 ;而对应的 4 0 5株 F2 和 182株 BC,GUS-株中分别有 99.3%和 99.5%出现了枯心。本实验的结果表明 ,应用杂交、回交常规育种方法将克螟稻的抗虫基因导入到新的推广水稻品种是可行的 ;同时也反映了报告基因 GUS仍可作为检测 cryl Ab的简便而有效的手段。
The resistance of Chilo suppressalis to crossbreds of japonica Bt transgenic rice “Kedaodao” and indica and japonica was studied by rapid identification of indoor leaves and field resistance. The results showed that plants that showed high resistance to Chilo suppressalis appeared in the crosses between indica and japonica rice (F1, F2 and BC1). In vitro leaf method, four combinations of 120 F1 hybrids and 88 GUS positive (GUS +) BC1 strains were similar to 30 Ke Kedao rice parents, 2 days of the mortality of the first larvae hatched larvae as high as 10 0. 0% respectively. However, the mortality of the newly hatched larvae of the borer was only 8.33% -2.28% within 4 days when fed with the corresponding combination of GUS-negative and non-transgenic parent leaves. In addition, in the experimental field where no pesticides toxic to Lepidoptera were applied, 8 combinations of 707 GUS + F2 plants and 7 combinations of 177 plants of GUS + BC1 were surveyed and only about 1% of the plants withered And white spike; while the corresponding 4 0 5 F2 and 182 BC, GUS-strains were 99.3% and 99.5% of the withered. The results of this experiment showed that it is feasible to introduce the insect-resistant gene of Kemingdao into new rice varieties by crossing and backcrossing conventional breeding methods. At the same time, the reporter gene GUS can be used as a convenient and effective method for detecting cryl Ab s method.