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目的：研究ｂａｘ过量表达对Ｂｕｒｋｉｔ淋巴瘤细胞株细胞程序性死亡的效应及其机理。方法：亚克隆构建可过量表达ｂａｘα的真核表达载体，通过脂质体转染法导入Ｒａｊｉ细胞株中。ＰＣＲ及免疫荧光定量鉴定ｂａｘα在Ｒａｊｉ细胞株中的表达；流式细胞仪检测凋亡率。结果：成功构建ｐＳＦＦＶｂａｘαＳ和ｐＳＦＦＶｂａｘαＡＳ两种质粒，转染Ｒａｊｉ细胞后，测得转染ｐＳＦＦＶｂａｘαＳ的Ｒａｊｉ细胞的ｂａｘα表达量明显高于转染空载ｐＳＦＦＶｎｅｏ质粒的Ｒａｊｉ细胞株。φ（胎牛血清）＝０．０１诱导两种Ｒａｊｉ细胞４８ｈ，Ｒａｊｉｂａｘα的凋亡率为４８．６％，Ｒａｊｉ０的凋亡率为６．４％。结论：ｂａｘα在Ｒａｊｉ细胞中的加强表达可明显促进细胞的凋亡。
Objective: To study the effect of bax overexpression on apoptosis of Burkit’s lymphoma cell line and its mechanism. Methods: The eukaryotic expression vector overexpressing baxα was subcloned and introduced into Raji cells by lipofection. PCR and immunofluorescence quantitative identification of baxα expression in Raji cell lines; apoptosis rate was detected by flow cytometry. Results: Two plasmids, pSFFVbaxαS and pSFFVbaxαAS, were constructed successfully. After transfected into Raji cells, the expression of baxα in Raji cells transfected with pSFFVbaxαS was significantly higher than that in pSFFV neo plasmid Raji cell line. φ (fetal bovine serum) = 0.01 induced two kinds of Raji cells 48h, Raji baxα apoptosis rate was 48.6%, Raji 0 apoptosis rate was 6.4%. Conclusion: Enhanced expression of baxα in Raji cells can significantly promote cell apoptosis.