目的通过建立一种新的手术模型,实现对脑脊液(CSF)的多次采集。方法研究Beagle犬头颅结构,定位手术钻孔,手术穿刺、埋管采集脑脊液。结果动物手术存活率为80%,模型成功率为60%。结论新模型设计合理,有利于多次采集,此方法可成为Beagle犬脑脊液采集新手段。 Objective To establish a new surgical model to achieve multiple acquisition of cerebrospinal fluid (CSF). Methods The beagle dogs’ skull structure, positioning surgery drilling, surgical puncture and buried tube were used to collect cerebrospinal fluid. Results The survival rate of animal surgery was 80% and the model success rate was 60%. Conclusion The new model is reasonable and is conducive to multiple acquisitions. This method can be a new method for collecting cerebrospinal fluid in Beagle dogs.