建立了薄层色谱 -荧光分光光度法测定葛根中葛根素含量的方法。以甲醇为溶剂进行提取 ,以氯仿-甲醇 -水 ( 7∶ 4∶ 0 .2 5)展开 ,刮下斑点后用甲醇洗脱 ,在 Ex=355nm ,Em=4 60 nm处测定荧光强度 ,线性范围为 0 .0 1～ 0 .0 8μg/ ml( r=0 .9998) ,平均回收率为 98.0 % ,RSD <5%。 A method for the determination of puerarin in Radix Puerariae by TLC-fluorescence spectrophotometry was established. Methanol was used as the solvent for extraction, and chloroform-methanol-water (7:4:0.25) was developed. The spots were scraped off and eluted with methanol. The fluorescence intensity was measured at Ex=355 nm and Em=460 nm. The range was from 0.01 to 0.8 mg/ml (r=0.9998), the average recovery was 98.0 %, RSD <5%.