论文部分内容阅读
1植物名称肿节石斛(Dendrobium pendulumRoxb.)。2材料类别成熟种子。3培养条件以N6和MS为基本培养基。种子萌发培养基:(1)N6;(2)MS;(3)1/2MS;(4)VW(Vacin-Went培养基);(5)MS+椰乳100 mg·L~(-1)(单位下同)。增殖与分化成苗培养基:(6)MS+6-BA 0.2+NAA 0.2。生根壮苗培养基:(7)MS+NAA 0.5;(8)MS+土豆泥100。上述培养基均附加30 g·L~(-1)蔗糖和6 g·L~(-1)琼脂,pH 5.5～5.8。培养温度为(24±2)℃;光照强度20～40μmol·m~(-2)·s~(-1),光照时间12 h·d~(-1)。
1 plant name Dendrobium pendulumRoxb. 2 material categories mature seeds. 3 culture conditions to N6 and MS as the basic medium. Seed germination medium: (1) N6; (2) MS; (3) 1/2 MS; (4) VW (Vacin-Went medium); The same below). Proliferation and differentiation into seedling medium: (6) MS + 6-BA 0.2 + NAA 0.2. Rooting strong seedling medium: (7) MS + NAA 0.5; (8) MS + mashed potatoes 100. The above medium was supplemented with 30 g · L -1 sucrose and 6 g · L -1 agar at pH 5.5-5.8. The culture temperature was (24 ± 2) ℃, light intensity was 20-40 μmol · m -2 · s -1, and illumination time was 12 h · d -1.