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目的探讨帕金森病(PD)异动症的产生与黑质损伤程度之间的关系。方法通过6-羟基多巴立体定向注射至大鼠前脑内侧束建立PD模型,左旋多巴甲酯腹腔注射治疗(25 mg·kg~(-1)·d~(-1),每天2次)21 d,评估异常不自主运动的情况；然后取中脑,行酪氨酸羟化酶免疫组织化学染色,观察黑质多巴胺能神经元的损伤程度；异常不自主运动积分与黑质损伤程度之间的关系应用Excel软件的S方程分析进行评价。结果25只大鼠经阿朴吗啡诱导后有10只旋转频率＞7次/ min,被认为是成功PD模型；经左旋多巴治疗后,10只PD大鼠中有8只出现了异常不自主运动,包括刻板动作和对侧旋转行为,并且每只大鼠的异常不自主运动均不同；异常不自主运动在黑质酪氨酸羟化酶阳性神经元的损伤达90%以上的PD大鼠中出现；黑质损伤程度和异常不自主运动的发生及程度呈正相关。结论黑质损伤严重程度在PD患者出现异动症中可能起重要作用。
Objective To investigate the relationship between the occurrence of Parkinson’s disease (PD) dyskinesia and the degree of nigral lesion. Methods PD model was established by stereotactic injection of 6-hydroxydopamine into the medial forebrain of rat forebrain. L-dopa methyl ester was administered intraperitoneally (25 mg · kg -1 · d -1) twice a day ) 21 d, to assess the situation of abnormal involuntary movement; then take the brain, line tyrosine hydroxylase immunohistochemical staining to observe the degree of nigral dopaminergic neuron damage; abnormal involuntary movement integral and the degree of nigral injury The relationship between the use of Excel software S-equation analysis to evaluate. Results 25 rats were induced by apomorphine 10 rotation frequency> 7 times / min, is considered a successful model of PD; after levodopa treatment, 8 out of 10 PD rats showed abnormal involuntary Exercise, including stereotyped movements and contralateral rotation behavior, and each rat’s abnormal involuntary movements are different; abnormal involuntary movement of substantia nigra tyrosine hydroxylase positive neurons damage more than 90% of PD rats ; There was a positive correlation between the degree of nigral lesion and the occurrence and degree of abnormal involuntary movements. Conclusion The severity of nigral lesion may play an important role in the development of dyskinesia in PD patients.