论文部分内容阅读
目的:研究提取-共沸精馏耦合工艺(water extraction coupling rectification,WER)提取挥发油制备颈复康颗粒对实验性体内、外血栓形成、血液黏度及脑血流量的影响,对WER工艺制备的颈复康颗粒进行药理学评价。方法:取SD大鼠40只,随机分成5组,每组8只。即颈复康颗粒高、低剂量组(12,6 g.kg-1)、WER工艺颈复康颗粒高、低剂量组(12,6 g.kg-1)及空白对照组,各组连续ig给药7 d。大鼠体外血栓形成:末次给药后2 h,自腹腔动脉取血,置体外血栓形成仪上测量血栓长度、湿重、干重;大鼠体内血栓形成:末次给药后2 h麻醉大鼠,分离颈总动脉,观察记录体内血栓形成时间;自腹腔静脉取血测定大鼠血小板聚集率、高切、中切、低切下全血黏度及血浆黏度;取健康成年家犬30只,分组及给药等同前,观察记录用药前后家犬脑血流量(cerebral blood flow,CBF)及脑血管阻力(cerebral vascular resistance,CVR),分析WER工艺与传统工艺制备的颈复康颗粒对上述各项指标的影响。结果:WER工艺提取挥发油制备的颈复康颗粒高、低剂量组均可显著延缓大鼠血栓形成时间(OT延长率为33.34%,18.87%),降低大鼠体外血栓长度、湿重和干重,降低血小板聚集率及全血黏度和血浆黏度(血小板5min聚集阻抗值分别为6.15Ω,7.24Ω),在末次给药后30～90 min均可增加家犬脑血流量,且与传统工艺颈复康颗粒同剂量组比较差异有显著性。结论:WER工艺提取挥发油制备的颈复康颗粒较传统工艺制备的颈复康颗粒具有更好的活血化瘀作用。
OBJECTIVE: To study the effect of extraction of essential oil from Jingrongkang granule on the in vitro and in vivo thrombosis, blood viscosity and cerebral blood flow by extraction-azeotropic distillation coupled process (WER) Fukang granules for pharmacological evaluation. Methods: Forty SD rats were randomly divided into 5 groups with 8 rats in each group. (12,6 g.kg-1), high-dose and low-dose group (12,6 g.kg-1) and blank control group ig administration 7 d. Rat in vitro thrombosis: 2 h after the last administration, blood was drawn from the celiac artery, and thrombus length, wet weight and dry weight were measured on an extracorporeal thrombosis instrument. Thrombus formation in rats: 2 h after the last administration, anesthetized rats , The common carotid arteries were isolated and the thrombosis time was recorded. The rate of platelet aggregation, high cut, middle cut, low cut whole blood viscosity and plasma viscosity were determined from the abdominal venous blood. 30 healthy adult dogs were divided into groups And before administration, the blood flow (CBF) and cerebral vascular resistance (CVR) in dogs were observed and recorded before and after treatment. The effects of JFK granules prepared by WER process and traditional process on the above items The impact of indicators. Results: The high and low doses of Jingfukang granule prepared by extraction of volatile oil by WER process can significantly delay the thrombosis time (33.34%, 18.87%) in rats, and reduce the thrombus length, wet weight and dry weight , Lower platelet aggregation rate and whole blood viscosity and plasma viscosity (platelet 5min aggregate impedance were 6.15Ω, 7.24Ω), 30 ~ 90min after the last administration can increase dog blood flow in dogs, and with the traditional technology neck Fukang granules with the same dose group was significantly different. CONCLUSION: Jingfukang granules prepared by extraction of volatile oil by WER process have better blood circulation and blood stasis effect than Jingfukang granules prepared by traditional process.