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【目的】通过观察中药复方脑力苏胶囊对血管性痴呆(VD)大鼠海马低氧诱导因子-1α(HIF-1α)阳性细胞表达的影响,探讨其改善VD大鼠认知障碍的作用机制。【方法】选用雄性SD大鼠90只,随机分为假手术组,模型组,脑力苏高、低剂量组(剂量分别为2.5、1.25 g.kg-1.d-1),喜得镇组(剂量为0.6 mg.kg-1.d-1);采用双侧颈总动脉结扎法复制VD大鼠模型,采用Morris水迷宫测试法检测VD大鼠学习、记忆改善状况,免疫组化法检测大鼠海马(CA1区)HIF-1α阳性细胞数。【结果】脑力苏高、低剂量组大鼠学习记忆能力显著改善(P<0.01),海马HIF-1α阳性细胞表达较模型组显著降低(P<0.01)。【结论】脑力苏胶囊治疗VD的作用与其能降低海马HIF-1α表达,提高缺血脑组织细胞内氧浓度有关。
【Objective】 The purpose of this study was to observe the effect of Naosu Su capsule on the expression of hypoxia inducible factor-1α (HIF-1α) -positive cells in hippocampus of vascular dementia (VD) rats and to explore its mechanism of improving cognition impairment in VD rats. 【Methods】 Ninety male Sprague-Dawley rats were randomly divided into three groups: sham-operation group, model group, high-dose and low-dose brain group (2.5 and 1.25 g.kg-1.d- (0.6 mg.kg-1.d-1). The bilateral common carotid artery ligation was used to duplicate the VD model in rats. Morris water maze test was used to detect learning and memory in VD rats. Immunohistochemistry The number of HIF-1α positive cells in the hippocampus of rats (CA1 area). [Results] The learning and memory ability of hippocampus and low dose group was significantly improved (P <0.01). The expression of HIF-1α positive cells in hippocampus was significantly lower than that in model group (P <0.01). 【Conclusion】 The effect of Naoli Su capsule in the treatment of VD is related to the decrease of hippocampal HIF-1α expression and the increase of intracellular oxygen concentration in ischemic brain tissue.