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目的探讨人牙周膜细胞在流体剪切力作用下淋巴样增强因子-1(lymphoid enhancer factor-1,LEF-1)m RNA的表达。方法对人牙周膜细胞进行原代培养;通过流体剪切力加力系统对人牙周膜细胞施加1.2 Pa的流体剪切力,作用0、0.5、2、4、8 h后,用RT-PCR方法检测细胞受力前后LEF-1 m RNA的表达变化。结果人牙周膜细胞在1.2 Pa的流体剪切力作用8 h后,LEF-1 m RNA的表达升高。结论流体剪切力刺激活化了人牙周膜细胞中LEF-1的转录,LEF-1 m RNA的表达升高,可能是在剪切应力作用下Wnt信号通路的应答反应。
Objective To investigate the expression of lymphoid enhancer factor-1 (LEF-1) m RNA in human periodontal ligament cells under fluid shear stress. METHODS: Human periodontal ligament cells were cultured in primary culture. Fluid shear force of 1.2 Pa was applied to human periodontal ligament cells by fluid shear force system. After 0, 0.5, 2, 4 and 8 h, -PCR method was used to detect the expression of LEF-1 m RNA before and after stress. Results Human periodontal ligament cells were exposed to fluid shear force of 1.2 Pa for 8 h, and the expression of LEF-1 mRNA increased. Conclusion Fluid shear stress stimulates the transcription of LEF-1 in human periodontal ligament cells and the expression of LEF-1 m RNA is increased, which may be the response of Wnt signaling pathway under the effect of shear stress.