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预先给小鼠灌胃不同剂量的京尼平甙后,以四氯化碳造模,通过测定小鼠血清中丙氨酸氨基转移酶(ALT)、天氡氨酸氨基转移酶(AST)以及肝脏内GSH的含量并制作组织病理切片,研究了京尼平甙对四氯化碳肝损伤小鼠的保护作用,结果表明,京尼平甙能抑制四氯化碳肝中毒小鼠血清中ALT和AST的活性以及增加肝脏内GSH的含量.然后研究了京尼平甙对正常小鼠肝微粒体内细胞色素P4502E1和细胞色素P4503A活性的影响以及肝脏内谷胱甘肽(GSH)系统的影响,表明京尼平甙对正常小鼠肝微粒体内CYP4502E1具有明显的抑制作用,并能增强肝脏内谷胱甘肽还原酶(GR)以及谷胱甘肽-S-转移酶活性.以上3个酶与自由基形成以及清除有关.图1表3参16
Different doses of genipinone were pre-administered to mice and modeled with carbon tetrachloride. Alanine aminotransferase (ALT), AST, and AST were measured in mouse serum. GSH content in the liver and histopathological sections were made to study the protective effect of genipin against carbon tetrachloride-induced liver injury in mice. The results showed that genipin could inhibit serum ALT in carbon tetrachloride liver poisoning mice. The activity of AST and the increase of GSH content in liver were studied. The effects of genipinone on the activity of cytochrome P4502E1 and cytochrome P4503A in hepatic microsomes of normal mice and the effect of glutathione (GSH) system in liver were studied. It was shown that genipinone has a significant inhibitory effect on CYP4502E1 in liver microsomes of normal mice, and it can enhance the activity of glutathione reductase (GR) and glutathione-S-transferase in the liver. The above three enzymes and Free radical formation and clearance related. Figure 1 Table 3 Reference 16