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糖尿病患者多出现胃肠道功能紊乱,如急性胃炎、胃溃疡,以及胃动力低下,胃排空延迟、胃内细菌过度滋长等,进一步导致肠道疾病。研究糖尿病胃内容物菌群结构变化对研究糖尿病发病机理及并发症治疗具有重要意义。该项研究采用变性梯度凝胶电泳技术,对10只2型糖尿病模型小鼠及10只正常对照小鼠进行胃内容物和粘膜样本菌群结构研究。结果表明,实验组小鼠与对照组小鼠胃内容物和粘膜菌群条带数、多样性指数、丰富度指数、均匀度指数与优势度指数均无显著差异,且相似度系数差异不明显。而特异条带测序结果显示正常小鼠胃内含乳杆菌,实验组小鼠胃内乳杆菌含量很低甚至检测不到。提示胃内乳杆菌与2型糖尿病密切相关。
More patients with diabetes gastrointestinal disorders, such as acute gastritis, gastric ulcer, and gastric motility, delayed gastric emptying, excessive growth of bacteria in the stomach, and further lead to intestinal diseases. It is of great significance to study the changes of the structure of diabetic stomach contents flora to study the pathogenesis and complications of diabetes mellitus. In this study, denaturing gradient gel electrophoresis (PAGE) was used to study the bacterial contents of gastric contents and mucosa in 10 type 2 diabetic mice and 10 normal control mice. The results showed that there was no significant difference in the bands, diversity index, evenness index, evenness index, and similarity coefficient between gastric mucosa and mucosa in experimental mice and control mice . The specific band sequencing results showed that the normal mice stomach Lactobacillus, the experimental group of mice stomach Lactobacillus content is very low or undetectable. Tip Lactobacillus in stomach and type 2 diabetes are closely related.