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为进一步评价日本血吸虫组分抗原(107-121kD)ELISA(FA-ELISA)检测血吸虫病人体内短程抗体方法的现场应用价值,本研究应用该法对急性、慢性、晚期血吸虫病人和健康人、毕支睾吸虫病人、姜片虫病人、肺吸虫病人以及100例已达标地区的自然人群进行检测,并与SEA-ELISA,及应用单抗检测循环抗原的方法对比。结果提示FA-ELISA具较高的敏感性和特异性。Youden招数可达0.96。特别对其他吸虫患者几乎无交叉反应,揭示该法具有较好的现场应用价值。
In order to further evaluate the field application value of the method for detecting short-range antibodies in Schistosoma japonicum antigen (107-121kD) ELISA (FA-ELISA) in schistosomiasis patients, this study applied this method to acute and chronic schistosomiasis patients and healthy people, Testis, gondii, paragonimiasis and 100 natural populations in established areas. The results were compared with SEA-ELISA and monoclonal antibody detection of circulating antigens. The results suggest FA-ELISA with high sensitivity and specificity. Youden tricks up to 0.96. Especially for other trematode patients almost no cross-reaction, revealing the law has better field application value.