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玉米种子纯度盐溶蛋白电泳鉴定的原理,就是把从玉米种子中提取的盐溶蛋白在聚丙烯酰胺凝胶的浓缩效应、分子筛效应和电泳分离的电荷效应作用下进行分离,通过染色显示蛋白质谱带类型。由于不同玉米品种遗传组成的不同,种子内所含的蛋白质种类也有差异,这些差异可利用电泳图谱加以鉴别,从而对种子真实性和品种纯度进行鉴定。此项技术重复性好,化学稳定性强,一直在玉米种子纯度检验中被广泛推广和应用。1.样品制备及配药过程
The principle of the maize seed purity salt-soluble protein electrophoresis identification is that the salt-soluble protein extracted from corn seeds is separated by the concentration effect of polyacrylamide gel, the molecular sieve effect and the charge effect of electrophoresis separation, and the protein spectrum Belt type. Due to the different genetic compositions of different maize varieties, there are also differences in the kinds of proteins contained in the seeds. These differences can be identified by using electrophoresis patterns to identify the seed authenticity and variety purity. This technique has good repeatability and strong chemical stability. It has been widely popularized and applied in the test of maize seed purity. 1. Sample preparation and dispensing process