目的探讨六味地黄多糖免疫药理活性的物质基础。方法运用现代药理学与植物化学紧密配合的方法，从六味地黄汤中逐步分离获得了六味地黄多糖ＣＡ４３Ｂ和Ｐ３。应用［３Ｈ］ＴｄＲ参入法和溶血空斑实验观察了其体外对小鼠脾细胞免疫功能的作用。结果ＣＡ４３Ｂ、Ｐ３体外单独使用对正常及快速老化模型ＳＡＭＰ８和ＳＡＭＲ１小鼠脾细胞增殖反应有明显的促进作用，但不能增强有丝分裂原ＣｏｎＡ诱导的脾细胞增殖反应，大剂量时有一定的抑制作用。ＣＡ４３Ｂ对绵羊红细胞（ＳＲＢＣ）体外诱导的正常小鼠脾抗体形成细胞生成反应亦有明显的促进作用，使抗体形成细胞（ＰＦＣ）的数目明显增加。结论ＣＡ４３Ｂ和Ｐ３可能为具有免疫调节作用的活性多糖。 Objective To investigate the material basis of the immunological pharmacological activity of polysaccharides from Rehmannia glutinosa. Methods Using the method of modern pharmacology and phytochemistry, the polysaccharides CA43B and P3 of Liu Wei Di Huang were gradually separated from Liu Wei Di Huang Tang. The [3H] TdR incorporation assay and hemolysis plaque assay were used to observe the effect of its in vitro on spleen cell immune function in mice. Results The use of CA43B and P3 alone could significantly promote the proliferation of SAMP8 and SAMR1 spleen cells in normal and rapid aging models, but could not enhance the mitogen-induced proliferation of splenocytes in ConA. The inhibitory effect. CA4-3B also significantly promoted the spleen antibody-forming cell formation induced by sheep red blood cells (SRBC) in normal mice, which increased the number of antibody-forming cells (PFCs). Conclusion CA43B and P3 may be active polysaccharides with immunomodulating effects.